Input the substrate sequence (example)

Please select the protease of interest from the dropdown menu to make the prediction: (help)

pepsin A (A01.001) cathepsin D (A01.009) cathepsin E (A01.010) rhizopuspepsin (A01.012) aspergillopepsin I (A01.016) necepsin-1 (A01.053) HIV-1 retropepsin (A02.001) cathepsin L (C01.032) cathepsin S (C01.034) cathepsin K (C01.036) falcipain-2 (C01.046) cathepsin B (C01.060) falcipain-3 (C01.063) cathepsin L3 ({Fasciola} sp.) (C01.129) calpain-1 (C02.001) calpain-2 (C02.002) caspase-1 (C14.001) caspase-3 (C14.003) caspase-7 (C14.004) caspase-6 (C14.005) caspase-8 (C14.009) matrix metallopeptidase-1 (M10.001) matrix metallopeptidase-8 (M10.002) matrix metallopeptidase-2 (M10.003) matrix metallopeptidase-9 (M10.004) matrix metallopeptidase-3 (M10.005) matrix metallopeptidase-7 (M10.008) matrix metallopeptidase-12 (M10.009) matrix metallopeptidase-13 (M10.013) membrane-type matrix metallopeptidase-1 (M10.014) astacin (M12.001) meprin alpha subunit (M12.002) meprin beta subunit (M12.004) LAST peptidase ({Limulus polyphemus}) (M12.032) LAST_MAM peptidase (M12.033) ADAMTS4 peptidase (M12.221) insulysin (M16.002) mitochondrial processing peptidase beta-subunit (M16.003) chymotrypsin A (cattle-type) (S01.001) granzyme B ({Homo sapiens}-type) (S01.010) kallikrein-related peptidase 5 (S01.017) elastase-2 (S01.131) cathepsin G (S01.133) granzyme A (S01.135) granzyme B, rodent-type (S01.136) thrombin (S01.217) plasmin (S01.233) kallikrein-related peptidase 4 (S01.251) glutamyl peptidase I (S01.269) lysyl peptidase (bacteria) (S01.280) lactocepin I (S08.019) kexin (S08.070) furin (S08.071) PCSK-1 peptidase (S08.072) PCSK-2 peptidase (S08.073) PCSK-4 peptidase (S08.074) PCSK-6 peptidase (S08.075) PCSK-5 peptidase (S08.076) PCSK-7 peptidase (S08.077) KPC2-type peptidase (S08.109) lactocepin-3 (S08.116) signal peptidase I (S26.001) signalase (animal) 21 kDa component (S26.010)

In cases where users want to predict multiple substrate sequences, we also provide a Java-based standalone version of Procleave,

which can be downloaded here: Click to Download

Citation:

If you use Procleave, please kindly cite our paper:

Song J, Ho BK, Tan H, Kleifeld O, Smith AI, Pike RN, Whisstock JC (2012). Procleave: a tool for protease-specific substrate cleavage site prediction using conditional random fields, submitted for publication