ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYLIAVPDL MAHATTELSS IGSALTAANA AAAVPTTGVL AAGADDVSAA VASLFAGHAQ 

        70         80         90        100        110        120 
AYQSLGGQVA AFHQQFLEGL RGASGAYVAA EAANVWPLQD LLGLINAPTQ ALLGRPLIGN 

       130        140        150        160        170        180 
GTNGTAANPN GGVGGLLIGN GGNGWNSTTG GAAGGNGGDA GLLGNGGTGG SGGAAGLVGN 

       190        200        210        220        230        240 
GGSGGSGGDG GTGGTADFLG GTQSTGGDGG GGGGGDAGVG GAGGALLGNG GAGGAGGNGG 

       250        260        270        280        290        300 
DGGGYVTSGN APRGGGNGGE AGDGGLGGSA GMWGAGGAGG AAGTGGAGIS GYPNGFGGSR 

       310        320 
GHRRQRRRWW HRWLVIRRRR CGRGRR 

Number of amino acids: 326

Molecular weight: 30000.52

Theoretical pI: 9.03

Amino acid composition: 
Ala (A)  50	 15.3%
Arg (R)  17	  5.2%
Asn (N)  18	  5.5%
Asp (D)  11	  3.4%
Cys (C)   1	  0.3%
Gln (Q)   9	  2.8%
Glu (E)   4	  1.2%
Gly (G) 104	 31.9%
His (H)   5	  1.5%
Ile (I)   7	  2.1%
Leu (L)  26	  8.0%
Lys (K)   0	  0.0%
Met (M)   3	  0.9%
Phe (F)   5	  1.5%
Pro (P)   8	  2.5%
Ser (S)  17	  5.2%
Thr (T)  17	  5.2%
Trp (W)   6	  1.8%
Tyr (Y)   5	  1.5%
Val (V)  13	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 17

Atomic composition:

Carbon      C	      1267
Hydrogen    H	      1988
Nitrogen    N	       420
Oxygen      O	       423
Sulfur      S	         4

Formula: C1267H1988N420O423S4
Total number of atoms: 4102

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40450
Abs 0.1% (=1 g/l)   1.348, assuming all pairs of Cys residues form cystines


Ext. coefficient    40450
Abs 0.1% (=1 g/l)   1.348, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.40
This classifies the protein as stable.



Aliphatic index: 66.38

Grand average of hydropathicity (GRAVY): -0.105